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KVCPharmaGROUP: The ELISA : ELISA is stand for Enzyme-linked immunosorbent assay. It is an immunological or serological test to check antigen present in the serum. It h...

ELISA is stand for Enzyme-linked immunosorbent assay. It is an immunological or serological test to check antigen present in the serum. It have 2 types Direct ELISA or Sandwich ELISA Test and Indirect ELISA test. For Direct ELISA test, it is used to diagnose various microbial infections (such as HIV, Hepatitis A and C, Cholera Vibrio, Helicobacter pylori,...), cancer and other immunological disorders. Different from Direct ELISA, Indirect ELISA is used to diagnosed Hantavirus, rubella virus, toxoplasma.... Procedure of Sandwich ELISA Pour known antigen of specific antibodies on well containing micro titer plate and wait for 2-3 hours or over night then wash with buffer to remove unbound antibodies.  Add patient serum specimen on the well and wait for 1-2 hours and then wash with buffer.  Add Enzyme labelled (linked or conjugated) antibodies on the well and wash with buffer.  Finally, add the substrate (colorless substance) on the well and wait for 1-2 hours to observe the react

ELISA test is stand for Enzyme-linked immunosorbent Assay which also known as Enzyme immunoassay (EIA), contains an enzyme-antibody complex that can be used as a color tracer for antigen-antibody reactions. The enzymes used most often are horseradish and alkaline phosphatase, both of which release a dye (chromogen) when exposed to their substrate. This technique also relies on a solid support such as a plastic micro titer plate that can absorb (attract on its surface) the reactants.           The Indirect ELISA test can detect antibodies in a serum sample. As with other indirect test, the final positive reaction is achieved by means of an antibody-antibody reaction. The indicator antibody is complexed to an enzyme that produces a color change with positive serum sample. The starting reactant is a known antigen that is absorbed to the surface of a well. To this, unknown serum is added. After rinsing, an enzyme-Ab reagent that can react with the unknown test antibody is placed in the we

Elisa stand for Enzyme-linked immunosorbent Assay which also known as enzyme immunoassay (EIA), contains an enzyme-antibody complex that can be used as a color tracer for antigen-antibody reaction. The enzymes used most often are horseradish peroxidase and alkaline phosphatase, both of which release a dye (chromogen) when exposed to their substrate. This technique also relies a solid support such as a plastic micro titer plate that can absorb (attract on its surface) the reactants.

ក្រមសីលធម៌ឱសថការី ១​​.​ និយមន័យក្រមសីលធម៌វិជ្ជាជីវៈឱសថ? ចំ​​ៈ គឺជាកាតព្វកិច្ចដែលឱសថការីត្រូវបំេពញ ចំពោះអតិថិជន សហការី អ្នកផ្តល់សេវា​ខាភិបាល និស្សិត វិជ្ជាជីវៈ និង​ អាជ្ញាធរមានសមត្ថកិច្ចក្នុងការអនុវត្តវិជ្ជាជីវៈឱសថ។​ ២. លក្ខណៈខុសគ្នារវាង ក្រមសីមធម៌វិជ្ជាជីវៈ​ និង​និតិក្រម? ចំៈ​ មាន៣ចំនុចៈ - ការរៀបចំ៉ ( development) ៖ ក្រមសីលធម៌ត្រូវបានរៀបចំដោយគណៈឱសថការីកម្ពុជា ( pharmacy professional ethic) រីនិតិក្រមត្រូវបានរៀបចំដោយស្ថាប័ននិតិបញ្ញត្តិ ឬស្ថាប័ននិតិប្រតិបត្តិ - ដែននៃការអនុវត្ត ( the field of implementation) ៖ ក្រមសីធម៌វិជ្ជាជីវៈត្រូវអនុវត្តចំពោះតែឱសថការីណាដែលប្រកបវិជ្ជាជីវៈឱសថ រីនិតិក្រមត្រូវអនុវត្តចំពោះរូបវ័ន្តបុគ្គល ( Physical person) និងនិតិបុគ្គល (ស្ថាប័នឬក្រសួង.. ) - ទោសបញ្ញត្តិ ការប្រព្រឹត្តល្មើសនិងក្រមសីលធម៌វិជ្ជាជីវៈ​​ ត្រូវទទួលទោសដែលមានលក្ខណៈជាអង្គការវិន័យ​ ដែលវិនិច្ឆ័យដោយ ក្រុមប្រឹក្សាគណៈឱសថការី​ ដូចជាការស្តីបន្ទោស ការប្រមានជាលាយ លក្ខអក្សរ ការផ្ជួរសមាជិកភាពជាបណ្តោះ អាសន្ន និង​ការដកហូតសមាជិកភាពជាអចិន្ត្រៃ៌។​ រីការប្រព្រឹត្តល្មើសនិងនិតិក្រមត្រូវផ្តន្ទាទោសដែលមានលក្ខណៈជា