Elisa stand for Enzyme-linked immunosorbent Assay which also known as enzyme immunoassay (EIA), contains an enzyme-antibody complex that can be used as a color tracer for antigen-antibody reaction. The enzymes used most often are horseradish peroxidase and alkaline phosphatase, both of which release a dye (chromogen) when exposed to their substrate. This technique also relies a solid support such as a plastic micro titer plate that can absorb (attract on its surface) the reactants.
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